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Two photon microscopy

Two-photon excitation microscopy is a fluorescence imaging technique that allows sub-micrometer resolution imaging, reducing overall photobleaching and photodamage.

We implemented a scanlsess two-photon microscope (SLM-2PM) that employs a diffractive Liquid Crystal On Silicon-Spatial Light Modulator (LCOS-SLM) to both acquire two-photon images and to collect fluorescence signals from several neurons simultaneously, while maintaining the single-cell resolution.

We perform two-photon calcium imaging experiments on acute cerebellar slices bulk loaded with Fura-2 AM. We acquire stimulus-induced calcium signals from different neuronal types constituting the cerebellar circuit in order to gain insight into its intertwined and complex dynamics.